Real-Time Optical Tracking of Protein Corona Formation on Single Nanoparticles in Serum.

ABSTRACT: The formation of a protein corona, where proteins spontaneously adhere to the surface of nanomaterials in biological environments, leads to changes in their physicochemical properties and subsequently affects their intended biomedical functionalities. Most current methods to study protein corona formation are ensemble-averaging and either require fluorescent labeling, washing steps, or are only applicable to specific types of particles. Here we introduce real-time all-optical nanoparticle analysis by scattering microscopy (RONAS) to track the formation of protein corona in full serum, at the single-particle level, without any labeling. RONAS uses optical scattering microscopy and enables real-time and in situ tracking of protein adsorption on metallic and dielectric nanoparticles with diff

SUBMITTER: Dolci M 

PROVIDER: S-EPMC10604089 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature