Genetic and Pharmacological Blockade of Sigma-1 Receptors Attenuates Inflammation-Associated Hypersensitivity during Acute Colitis in CD1 Mice.

ABSTRACT: Sigma-1 receptors (σ₁Rs) are implicated in nociception, including pain sensitization, and inflammation. We assessed the role of σ₁Rs on acute colitis-associated hypersensitivity using both genetic (constitutive knockout) and pharmacological blockade of the receptor. Colitis was induced in CD1 wild-type (WT) and σ₁R KO mice (exposure to dextran sodium sulfate, 3%). A von Frey test was used to assess referred mechanosensitivity (abdominal and plantar withdrawal responses). The effects of the selective σ₁R antagonists BD1063 and E-52862 were also assessed in WT animals. The expression of immune and sensory-related markers (RT-qPCR, Western blot) was assessed in the colon and lumbosacral spinal cord. The genetic ablation or pharmacological blockade o

SUBMITTER: Lopez-Estevez S 

PROVIDER: S-EPMC10604167 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature