Clonal and Scalable Endothelial Progenitor Cell Lines from Human Pluripotent Stem Cells.

ABSTRACT: Human pluripotent stem cells (hPSCs) can be used as a renewable source of endothelial cells for treating cardiovascular disease and other ischemic conditions. Here, we present the derivation and characterization of a panel of distinct clonal embryonic endothelial progenitor cells (eEPCs) lines that were differentiated from human embryonic stem cells (hESCs). The hESC line, ESI-017, was first partially differentiated to produce candidate cultures from which eEPCs were cloned. Endothelial cell identity was assessed by transcriptomic analysis, cell surface marker expression, immunocytochemical marker analysis, and functional analysis of cells and exosomes using vascular network forming assays. The transcriptome of the eEPC lines was compared to various adult endothelial lines as well as vario

SUBMITTER: Lee J 

PROVIDER: S-EPMC10604251 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature