PREB inhibits the replication of prototype foamy virus by affecting its transcription.

ABSTRACT:

Background

Foamy viruses (FVs) are unique nonpathogenic retroviruses, which remain latent in the host for a long time. Therefore, they may be safe, effective gene transfer vectors. In this study, were assessed FV-host cell interactions and the molecular mechanisms underlying FV latent infection.

Methods

We used the prototype FV (PFV) to infect HT1080 cells and a PFV indicator cell line (PFVL) to measure virus titers. After 48 h of infection, the culture supernatant (i.e., cell-free PFV particles) and transfected cells (i.e., cell-associated PFV particles) were harvested and incubated with PFVL. After another 48 h, the luciferase activity was used to measure virus titers.

Results

Through transcriptomics sequencing, we found that PREB mRNA expression was significantly u

SUBMITTER: Zhang J 

PROVIDER: S-EPMC10604407 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature