Artificial Vision: The High-Frequency Electrical Stimulation of the Blind Mouse Retina Decay Spike Generation and Electrogenically Clamped Intracellular Ca²⁺ at Elevated Levels.

ABSTRACT:

Background

The electrical stimulation (stim) of retinal neurons enables blind patients to experience limited artificial vision. A rapid response outage of the stimulated ganglion cells (GCs) allows for a low visual sensation rate. Hence, to elucidate the underlying mechanism, we investigated different stim parameters and the role of the neuromodulator calcium (Ca²⁺).

Methods

Subretinal stim was applied on retinal explants (blind rd1 mouse) using multielectrode arrays (MEAs) or single metal electrodes, and the GC activity was recorded using Ca²⁺ imaging or MEA, respectively. Stim parameters, including voltage, phase polarity, and frequency, were investigated using specific blockers.

Results

At lower stim frequencies (<5 Hz), GCs responded s

SUBMITTER: Peiroten L 

PROVIDER: S-EPMC10604554 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature