Hypoxic Extracellular Matrix Preserves Its Competence after Expansion of Human MSCs under Physiological Hypoxia In Vitro.

ABSTRACT: Tissue-relevant O₂ levels are considered as an important tool for the preconditioning of multipotent mesenchymal stromal cells (MSCs) for regenerative medicine needs. The present study investigated the quality and functions of the extracellular matrix (ECM) of MSCs under low O₂ levels. Human adipose tissue-derived MSCs were continuously expanded under normoxia (20% O₂, N) or "physiological" hypoxia (5% O₂, Hyp). Decellularized ECM (dcECM) was prepared. The structure of the dcECM was analyzed using confocal laser and scanning electron microscopy. Collagen, dcECM-N, and dcECM-Hyp were recellularized with MSC-N and further cultured at normoxia. The efficacy of adhesion, spreading, growth, osteogenic potential, and paracrine activity of recellularize

SUBMITTER: Matveeva D 

PROVIDER: S-EPMC10604705 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature