Hydroxyl Radical vs. One-Electron Oxidation Reactivities in an Alternating GC Double-Stranded Oligonucleotide: A New Type Electron Hole Stabilization.

ABSTRACT: We examined the reaction of hydroxyl radicals (HO^•) and sulfate radical anions (SO₄^•-), which is generated by ionizing radiation in aqueous solutions under anoxic conditions, with an alternating GC doubled-stranded oligodeoxynucleotide (ds-ODN), i.e., the palindromic 5'-d(GCGCGC)-3'. In particular, the optical spectra of the intermediate species and associated kinetic data in the range of ns to ms were obtained via pulse radiolysis. Computational studies by means of density functional theory (DFT) for structural and time-dependent DFT for spectroscopic features were performed on 5'-d(GCGC)-3'. Comprehensively, our results suggest the addition of HO^• to the G:C pair moiety, affording the [8-HO-G:C]^• detectable adduct. The previous repor

SUBMITTER: Masi A 

PROVIDER: S-EPMC10605094 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature