Evening Primrose Extract Modulates TYMS Expression via SP1 Transcription Factor in Malignant Pleural Mesothelioma.

ABSTRACT:

Purpose

To determine the mechanism of EPE in downregulating TYMS in MPM cancer.

Methods

The TYMS mRNA expression with epithelial-to-mesenchymal transition biomarkers and nuclear factor SP1 was assessed using the GEO database in a data set of MPM patients (GSE51024). Invasive MPM cell lines were in vitro models for the investigation of TYMS expression after EPE treatment. The tyms promoter SP1 binding sequences were determined using Genomatix v 3.4 software Electrophoretic mobility shift and dual-luciferase reporter assays revealed specific SP1 motifs in the interaction of EPE and reference compounds. Chromatin immunoprecipitation and Re-ChIP were used for the co-occupancy study.

Results

In MPM patients, a positive correlation of overexpressed TYMS with mesenchy

SUBMITTER: Chmielewska-Kassassir M 

PROVIDER: S-EPMC10605291 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature