Transcriptomics integrated with metabolomics reveals the effect of cold stress on rice microspores.

ABSTRACT:

Background

Microspore culture is one of the important biotechnological tools in plant breeding. The induction of microspore embryogenesis is a critical factor that affects the yield of microspore-derived embryo productions. Cold treatment has been reported to reprogram the gametophytic pathway in various plant species. However, the exact mechanism(s) underlying the effect of cold pre-treatment of floral buds on the efficiency of ME is still not clear.

Results

In this study, the effects of cold stress on the microspore totipotency of rice cultivar Zhonghua 11 were investigated. Our results revealed that a 10-day cold treatment is necessary for microspore embryogenesis initiation. During this period, the survival rate of microspores increased and reached a peak at 7 days post

SUBMITTER: Li Y 

PROVIDER: S-EPMC10605337 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature