PACAP-Sirtuin3 alleviates cognitive impairment through autophagy in Alzheimer's disease.

ABSTRACT:

Background

Autophagy is vital in the pathogenesis of neurodegeneration. Thus far, no studies have specifically investigated the relationship between pituitary adenylate cyclase-activating polypeptide (PACAP) and autophagy, particularly in the context of Alzheimer's disease (AD). This study used in vitro and in vivo models, along with clinical samples, to explore interactions between PACAP and autophagy in AD.

Methods

AD model mice were administered 6 μl of 0.1 mg/ml PACAP liquid intranasally for 4 weeks, then subjected to behavioral analyses to assess the benefits of PACAP treatment. The underlying mechanisms of PACAP-induced effects were investigated by methods including real-time quantitative polymerase chain reaction, RNA sequencing, immunofluorescence, and western blotti

SUBMITTER: Wang Q 

PROVIDER: S-EPMC10605376 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature