Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.

ABSTRACT: Modern drug discovery relies on combinatorial screening campaigns to find drug molecules targeting specific disease-associated proteins. The success of such campaigns often relies on functional and structural information of the selected therapeutic target, only achievable once its purification is mastered. With the aim of bypassing the protein purification process to gain insights on the druggability, ligand binding, and/or characterization of protein-protein interactions, herein, we describe the Extract2Chip method. This approach builds on the immobilization of site-specific biotinylated proteins of interest, directly from cellular extracts, on avidin-coated sensor chips to allow for the characterization of molecular interactions via surface plasmon resonance (SPR). The developed method w

SUBMITTER: Paiva ACF 

PROVIDER: S-EPMC10605449 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature