Detecting Microsatellite Instability in Endometrial, Colon, and Stomach Cancers Using Targeted NGS.

ABSTRACT:

Purpose

To develop a method for testing the MSI based on targeted NGS.

Methods

Based on the results of previous studies, 81 microsatellite loci with high variability in MSI-H tumors were selected, and a method for calculating the MSI score was developed. Using the MSI score, we defined the MSI status in endometral (162), colon (153), and stomach (190) cancers. Accuracy of the MSI scores was evaluated by comparison with MMR immunohistochemistry for 137 endometrium (63 dMMR and 74 pMMR), 76 colon (29 dMMR and 47 pMMR), and 81 stomach (8 dMMR and 73 pMMR) cancers.

Results

Classification of MSS and MSI-H tumors was performed with AUC (0.99), sensitivity (92%), and specificity (98%) for all tumors without division into types. The accuracy of MSI testing in endometrial canc

SUBMITTER: Boyarskikh U 

PROVIDER: S-EPMC10605658 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature