Injectable In Situ Crosslinking Hydrogel for Autologous Fat Grafting.

ABSTRACT: Autologous fat grafting is hampered by unpredictable outcomes due to high tissue resorption. Hydrogels based on enzymatically pretreated tunicate nanocellulose (ETC) and alginate (ALG) are biocompatible, safe, and present physiochemical properties capable of promoting cell survival. Here, we compared in situ and ex situ crosslinking of ETC/ALG hydrogels combined with lipoaspirate human adipose tissue (LAT) to generate an injectable formulation capable of retaining dimensional stability in vivo. We performed in situ crosslinking using two different approaches; inducing Ca²⁺ release from CaCO₃ microparticles (CMPs) and physiologically available Ca²⁺ in vivo. Additionally, we generated ex situ-crosslinked, 3D-bioprinted hydrogel-fat grafts. We found that in vi

SUBMITTER: Oskarsdotter K 

PROVIDER: S-EPMC10606883 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature