Challenges in Imaging Analyses of Biomolecular Condensates in Cells Infected with Influenza A Virus.

ABSTRACT: Biomolecular condensates are crucial compartments within cells, relying on their material properties for function. They form and persist through weak, transient interactions, often undetectable by classical biochemical approaches. Hence, microscopy-based techniques have been the most reliable methods to detail the molecular mechanisms controlling their formation, material properties, and alterations, including dissolution or phase transitions due to cellular manipulation and disease, and to search for novel therapeutic strategies targeting biomolecular condensates. However, technical challenges in microscopy-based analysis persist. This paper discusses imaging, data acquisition, and analytical methodologies' advantages, challenges, and limitations in determining biophysical parameters expl

SUBMITTER: Etibor TA 

PROVIDER: S-EPMC10607852 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature