Hexa-Histidine, a Peptide with Versatile Applications in the Study of Amyloid-β(1-42) Molecular Mechanisms of Action.

ABSTRACT: Amyloid β (Aβ) oligomers are the most neurotoxic forms of Aβ, and Aβ(1-42) is the prevalent Aβ peptide found in the amyloid plaques of Alzheimer's disease patients. Aβ(25-35) is the shortest peptide that retains the toxicity of Aβ(1-42). Aβ oligomers bind to calmodulin (CaM) and calbindin-D28k with dissociation constants in the nanomolar Aβ(1-42) concentration range. Aβ and histidine-rich proteins have a high affinity for transition metal ions Cu²⁺, Fe³⁺ and Zn²⁺. In this work, we show that the fluorescence of Aβ(1-42) HiLyte^TM-Fluor555 can be used to monitor hexa-histidine peptide (His₆) interaction with Aβ(1-42). The formation of His₆/Aβ(1-42) complexes is also supported by docking results yielded by the MDockPeP Server. A

SUBMITTER: Salazar J 

PROVIDER: S-EPMC10609148 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature