Engineering Escherichia coli for Isobutanol Production from Xylose or Glucose-Xylose Mixture.

ABSTRACT: Aiming to overcome the depletion of fossil fuels and serious environmental pollution, biofuels such as isobutanol have garnered increased attention. Among different synthesis methods, the microbial fermentation of isobutanol from raw substrate is a promising strategy due to its low cost and environmentally friendly and optically pure products. As an important component of lignocellulosics and the second most common sugar in nature, xylose has become a promising renewable resource for microbial production. However, bottlenecks in xylose utilization limit its wide application as substrates. In this work, an isobutanol synthetic pathway from xylose was first constructed in E. coli MG1655 through the combination of the Ehrlich and Dahms pathways. The engineering of xylose transport and

SUBMITTER: Gu P 

PROVIDER: S-EPMC10609591 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature