Construction of Candida albicans Adhesin-Exposed Synthetic Cells for Preventing Systemic Fungal Infection.

ABSTRACT: The development of efficient fungal vaccines is urgent for preventing life-threatening systemic fungal infections. In this study, we prepared a synthetic, cell-based fungal vaccine for preventing systemic fungal infections using synthetic biology techniques. The synthetic cell EmEAP1 was constructed by transforming the Escherichia coli chassis using a de novo synthetic fragment encoding the protein mChEap1 that was composed of the E. coli OmpA peptide, the fluorescence protein mCherry, the Candida albicans adhesin Eap1, and the C-terminally transmembrane region. The EmEAP1 cells highly exposed the mChEap1 on the cell surface under IPTG induction. The fungal vaccine was then prepared by mixing the EmEAP1 cells with aluminum hydroxide gel and CpG. Fluorescence quantifica

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC10611093 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature