Novel Potent Autophagy Inhibitor Ka-003 Inhibits Dengue Virus Replication.

ABSTRACT: Every year, dengue virus (DENV) affects millions of people. Currently, there are no approved drugs for the treatment of DENV infection. Autophagy is a conserved degradation process that was shown to be induced by DENV infection and required for optimal DENV replication. The modulation of autophagy is, therefore, considered an attractive target to treat DENV infection. This study carried out a high-content image screen analysis using Crispr-Cas9 GFP-LC3 knocked-in HeLa cells of a compound library synthesized from or inspired by natural products and their biocongener precursors to discover novel autophagy inhibitors. The screen identified Ka-003 as the most effective compound for decreasing the number of autophagic vacuoles inside cells upon autophagy induction. Ka-003 could inhibit autophag

SUBMITTER: Limthongkul J 

PROVIDER: S-EPMC10611120 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature