PACAP regulates VPAC1 expression, inflammatory processes and lipid homeostasis in M1- and M2-macrophages.

ABSTRACT:

Background

Pituitary adenylate cyclase-activating polypeptide (PACAP) acts as an anti-atherogenic neuropeptide and plays an important role in cytoprotective, as well as inflammatory processes, and cardiovascular regulation. Therefore, the aim of this study is to investigate the regulatory effects of PACAP and its receptor VPAC1 in relation to inflammatory processes and lipid homeostasis in different macrophage (MΦ) subtypes.

Methods

To investigate the role of PACAP deficiency in the pathogenesis of atherosclerosis under standard chow (SC) or cholesterol-enriched diet (CED) in vivo, PACAP^-/- mice were crossbred with ApoE^-/- to generate PACAP^-/-/ApoE^-/- mice. Lumen stenosis in the aortic arch and different MΦ-subtypes were ana

SUBMITTER: Witzel R 

PROVIDER: S-EPMC10611464 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature