Occurrence and characterization of plasmids carrying tmexCD1-toprJ1, bla_DHA-1, and bla_CTX-M-127, in clinical Klebsiella pneumoniae strains.

ABSTRACT:

Objective

Today, the emergence of Klebsiella pneumoniae with the tmexCD1-toprJ1 gene cassette in patients has presented a significant clinical challenge.

Methods

To present the detailed genetic features of the tmexCD1-toprJ1 gene cassette of K. pneumoniae strain F4_plasmid pA, the whole bacterial genome was sequenced by Illumina and nanopore platforms, and mobile genetic elements related to antibiotic resistance genes were analyzed with a series of bioinformatics methods.

Results

K. pneumoniae strain F4 was determined to be a class A+C beta-lactamase, and was resistant to routinely used antibiotics, especially tigecycline, because of the oqxAB gene localized on the F4_chromosome and tmexCD1-toprJ1 on F4_plasmid A. After

SUBMITTER: Qu Y 

PROVIDER: S-EPMC10611489 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature