Novel alleles in the era of next-generation sequencing-based HLA typing calls for standardization and policy.

ABSTRACT: Next-Generation Sequencing (NGS) has transformed clinical histocompatibility laboratories through its capacity to provide accurate, high-throughput, high-resolution typing of Human Leukocyte Antigen (HLA) genes, which is critical for transplant safety and success. As this technology becomes widely used for clinical genotyping, histocompatibility laboratories now have an increased capability to identify novel HLA alleles that previously would not be detected using traditional genotyping methods. Standard guidelines for the clinical verification and reporting of novelties in the era of NGS are greatly needed. Here, we describe the experience of a clinical histocompatibility laboratory's use of NGS for HLA genotyping and its management of novel alleles detected in an ethnically-diverse popula

SUBMITTER: Tran JN 

PROVIDER: S-EPMC10611506 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature