Recombinant human diamine oxidase prevents hemodynamic effects of continuous histamine infusion in guinea pigs.

ABSTRACT:

Objective

To test whether recombinant human diamine oxidase (rhDAO) with a mutated heparin-binding motif (mHBM), which shows an increased alpha-distribution half-life, prevents histamine-induced hemodynamic effects.

Material

Thirty-eight female guinea pigs were either pretreated with rhDOA_mHBM or buffer.

Treatment and methods

Guinea pigs received a continuous infusion of histamine. Heart rate (HR), body core temperature and mean arterial pressure (MAP) were measured and blood was collected.

Results

Continuous intravenous infusion of 8 µg/kg/min histamine increased mean peak plasma histamine levels from 5 (± 0.3 SEM) to 28 ng/mL (± 4.9 SEM) after 30 min but had no effect on oxygen saturation. Guinea pigs pretreated with 4 mg/kg rhDAO_mHBM showed lower mean HR (

SUBMITTER: Weiss-Tessbach M 

PROVIDER: S-EPMC10611646 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature