Rapid Generation of Therapeutic Nanoparticles Using Cell-Free Expression Systems.

ABSTRACT: The surface modification of membrane-based nanoparticles, such as liposomes, polymersomes, and lipid nanoparticles, with targeting molecules, such as binding proteins, is an important step in the design of therapeutic materials. However, this modification can be costly and time-consuming, requiring cellular hosts for protein expression and lengthy purification and conjugation steps to attach proteins to the surface of nanocarriers, which ultimately limits the development of effective protein-conjugated nanocarriers. Here, the use of cell-free protein synthesis systems to rapidly create protein-conjugated membrane-based nanocarriers is demonstrated. Using this approach, multiple types of functional binding proteins, including affibodies, computationally designed proteins, and scFvs, can be

SUBMITTER: Peruzzi JA 

PROVIDER: S-EPMC10611898 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature