A novel transcriptional repressor specifically regulates xylanase gene 1 in Trichoderma reesei.

ABSTRACT:

Background

The well-known industrial fungus Trichoderma reesei has an excellent capability of secreting a large amount of cellulases and xylanases. The induced expression of cellulase and xylanase genes is tightly controlled at the transcriptional level. However, compared to the intensive studies on the intricate regulatory mechanism of cellulase genes, efforts to understand how xylanase genes are regulated are relatively limited, which impedes the further improvement of xylanase production by T. reesei via rational strain engineering.

Results

To identify transcription factors involved in regulating xylanase gene expression in T. reesei, yeast one-hybrid screen was performed based on the promoters of two major extracellular xylanase genes xyn1 and xyn2. A putative transcript

SUBMITTER: Xu W 

PROVIDER: S-EPMC10612264 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature