Greenness assessment of two chromatographic methods developed for the determination of Mupirocin in two binary mixtures along with its impurity.

ABSTRACT: Two simple, accurate and precise chromatographic methods have been developed and validated for estimating Mupirocin (MUP) in two binary mixtures. Mixture (1); with Fluticasone propionate (FLU) together with two of their impurities, namely; Pseudomonic acid-D (Pseud-D) and Fluticasone impurity C (FIC). Mixture (2); with Mometasone furoate (MF) along with Pseud-D impurity. High performance thin layer chromatography (HPTLC-densitometry) and high performance liquid chromatography (RP-HPLC) were the two proposed methods. In the HPTLC method, good separation of both mixtures was achieved by using HPTLC plates pre-coated with silica gel 60 F₂₅₄ as stationary phase and the mobile phase consisted of toluene: chloroform: ethanol at a ratio of (5: 4: 2, by volume). The detection was carrie

SUBMITTER: Galal MM 

PROVIDER: S-EPMC10612294 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature