Population dynamics of phenol-degrading bacteria in activated sludge determined by gyrB-targeted quantitative PCR.

ABSTRACT: A method for quantifying bacterial populations introduced into an activated-sludge microbial community is described. The method involves extraction of DNA from activated sludge, appropriate dilution of the extracted DNA with DNA extracted from nonintroduced activated sludge, PCR amplification of a gyrB gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the electrophoresed PCR product by densitometry. The adequacy of the method was examined by analyzing the population dynamics of two phenol-degrading bacteria, Pseudomonas putida BH and Comamonas sp. strain E6, that had been introduced into phenol-digesting activated sludge. The density of each of the two populations determined by the PCR method immediately after the introduction was consist

SUBMITTER: Watanabe K 

PROVIDER: S-EPMC106130 | biostudies-literature | 1998 Apr

REPOSITORIES: biostudies-literature