Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics.

ABSTRACT:

Background

There is no direct evidence of gut microbiota disturbance in children with gastroesophageal reflux disease (GERD). This study aimed to provide direct evidence and a comprehensive understanding of gut microbiota disturbance in children with GERD through combined metagenomic and metabolomic analysis.

Methods

30 children with GERD and 30 healthy controls (HCs) were continuously enrolled, and the demographic and clinical characteristics of the subjects were collected. First, 16S rRNA sequencing was used to evaluate differences in the gut microbiota between children with GERD and HC group, and 10 children with GERD and 10 children in the HC group were selected for metagenomic analysis. Nontargeted metabolomic analysis was performed using liquid chromatography/mass spec

SUBMITTER: Ye X 

PROVIDER: S-EPMC10613033 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature