Stem cell proliferation and differentiation during larval metamorphosis of the model tapeworm Hymenolepis microstoma.

ABSTRACT:

Background

Tapeworm larvae cause important diseases in humans and domestic animals. During infection, the first larval stage undergoes a metamorphosis where tissues are formed de novo from a population of stem cells called germinative cells. This process is difficult to study for human pathogens, as these larvae are infectious and difficult to obtain in the laboratory.

Methods

In this work, we analyzed cell proliferation and differentiation during larval metamorphosis in the model tapeworm Hymenolepis microstoma, by in vivo labelling of proliferating cells with the thymidine analogue 5-ethynyl-2'-deoxyuridine (EdU), tracing their differentiation with a suite of specific molecular markers for different cell types.

Results

Proliferating cells are ve

SUBMITTER: Montagne J 

PROVIDER: S-EPMC10614006 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature