Internucleotidic bond formation using H-phosphonamidate derivatives and acidic activators.

ABSTRACT: We previously developed a synthesis method for oligodeoxynucleotides using H-phosphonamidate derivatives as monomers. In this synthesis method, H-phosphonamidate monomers having a heterocyclic amino group as a leaving group reacts with an alcohol to form an internucleotidic H-phosphonate diester in pyridine without any additives upon heating. In this study, H-phosphonamidate reacted to form an internucleotidic linkage in the presence of acidic activators at room temperature. This finding is the first example of the activation of an amino group of an H-phosphonamidate derivative in the presence of acidic activators. In addition, the condensation reaction with acidic activators was accelerated with the use of pyridine as a solvent. Moreover, heterocyclic am

SUBMITTER: Tsurusaki T 

PROVIDER: S-EPMC10614043 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature