Assessment of stained direct cytology smears of breast cancer for whole transcriptome and targeted messenger RNA sequencing.

ABSTRACT:

Background

Rather than surgical resection, cytologic specimens are often used as first-line clinical diagnostic procedures due to higher safety, speed, and cost-effectiveness. Archival diagnostic cytology slides containing cancer can be equivalent to tissue biopsies for DNA mutation testing, but the accuracy of transcriptomic profiling by RNA sequencing (RNA-seq) is less understood.

Methods

This study compares the results from whole transcriptome RNA-seq and a targeted RNA-seq assay of stained cytology smears (CS) versus matched tumor tissue samples preserved fresh-frozen (FF) and processed as formalin-fixed paraffin-embedded (FFPE) sections. Cellular cytology scrapes from all 11 breast cancers were fixed and stained using three common protocols: Carnoy's (CS_C) or 95% ethan

SUBMITTER: Marczyk M 

PROVIDER: S-EPMC10614161 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature