Neutral sphingomyelinase inhibition promotes local and network degeneration in vitro and in vivo.

ABSTRACT:

Background

Cell-to-cell communication is vital for tissues to respond, adapt, and thrive in the prevailing milieu. Several mechanisms mediate intercellular signaling, including tunneling nanotubes, gap junctions, and extracellular vesicles (EV). Depending on local and systemic conditions, EVs may contain cargoes that promote survival, neuroprotection, or pathology. Our understanding of pathologic intercellular signaling has been bolstered by disease models using neurons derived from human pluripotent stems cells (hPSC).

Methods

Here, we used hPSC-derived retinal ganglion cells (hRGC) and the mouse visual system to investigate the influence of modulating EV generation on intercellular trafficking and cell survival. We probed the impact of EV modulation on cell survival by dec

SUBMITTER: Risner ML 

PROVIDER: S-EPMC10614343 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature