Identification of RNA reads encoding different channels in isolated rat ventricular myocytes and the effect of cell stretching on L-type Ca²⁺current.

ABSTRACT:

Background

The study aimed to identify transcripts of specific ion channels in rat ventricular cardiomyocytes and determine their potential role in the regulation of ionic currents in response to mechanical stimulation. The gene expression levels of various ion channels in freshly isolated rat ventricular cardiomyocytes were investigated using the RNA-seq technique. We also measured changes in current through Ca_V1.2 channels under cell stretching using the whole-cell patch-clamp method.

Results

Among channels that showed mechanosensitivity, significant amounts of TRPM7, TRPC1, and TRPM4 transcripts were found. We suppose that the recorded L-type Ca²⁺ current is probably expressed through Ca_V1.2. Furthermore, stretching cells by 6, 8, and 10 μ

SUBMITTER: Kamkin AG 

PROVIDER: S-EPMC10614344 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature