Serum anti-PCK1 antibody levels are a prognostic factor for patients with diabetes mellitus.

ABSTRACT:

Background

Autoantibodies develop in autoimmune diseases, cancer, diabetes mellitus (DM), and atherosclerosis-related diseases. However, autoantibody biomarkers have not been successfully examined for diagnosis and therapy.

Methods

Serological identification of antigens through recombinant cDNA expression cloning (SEREX) was used for primary screening of antigens. The cDNA product was expressed in bacteria and purified. Amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) was used to evaluate antibody levels in serum samples.

Results

Phosphoenolpyruvate carboxykinase 1 (PCK1) was recognized as an antigen by serum IgG antibodies in the sera of patients with atherosclerosis. AlphaLISA showed significantly higher serum antibody levels

SUBMITTER: Namiki T 

PROVIDER: S-EPMC10614393 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature