Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis.

ABSTRACT:

Introduction

Culturing cerebrovascular smooth muscle cells (CVSMCs) in vitro can provide a model for studying many cerebrovascular diseases. This study describes a convenient and efficient method to obtain mouse CVSMCs by enzyme digestion.

Methods

Mouse circle of Willis was isolated, digested, and cultured with platelet-derived growth factor-BB (PDGF-BB) to promote CVSMC growth, and CVSMCs were identified by morphology, immunofluorescence analysis, and flow cytometry. The effect of PDGF-BB on vascular smooth muscle cell (VSMC) proliferation was evaluated by cell counting kit (CCK)-8 assay, morphological observations, Western blotting, and flow cytometry.

Results

CVSMCs cultured in a PDGF-BB-free culture medium had a typical peak-to-valley growth pattern after approxim

SUBMITTER: Chang W 

PROVIDER: S-EPMC10614493 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature