Identification of a carbonic anhydrase-Rubisco complex within the alpha-carboxysome.

ABSTRACT: Carboxysomes are proteinaceous organelles that encapsulate key enzymes of CO₂ fixation-Rubisco and carbonic anhydrase-and are the centerpiece of the bacterial CO₂ concentrating mechanism (CCM). In the CCM, actively accumulated cytosolic bicarbonate diffuses into the carboxysome and is converted to CO₂ by carbonic anhydrase, producing a high CO₂ concentration near Rubisco and ensuring efficient carboxylation. Self-assembly of the α-carboxysome is orchestrated by the intrinsically disordered scaffolding protein, CsoS2, which interacts with both Rubisco and carboxysomal shell proteins, but it is unknown how the carbonic anhydrase, CsoSCA, is incorporated into the α-carboxysome. Here, we present the structural basis of carbonic anhydrase encapsulatio

SUBMITTER: Blikstad C 

PROVIDER: S-EPMC10614612 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature