A blue-shifted genetically encoded Ca²⁺ indicator with enhanced two-photon absorption.

ABSTRACT:

Significance

Genetically encoded calcium ion (Ca²⁺) indicators (GECIs) are powerful tools for monitoring intracellular Ca²⁺ concentration changes in living cells and model organisms. In particular, GECIs have found particular utility for monitoring the transient increase of Ca²⁺ concentration that is associated with the neuronal action potential. However, the palette of highly optimized GECIs for imaging of neuronal activity remains relatively limited. Expanding the selection of available GECIs to include new colors and distinct photophysical properties could create new opportunities for in vitro and in vivo fluorescence imaging of neuronal activity. In particular, blue-shifted variants of GECIs are expected to have enhanced two-photon

SUBMITTER: Aggarwal A 

PROVIDER: S-EPMC10614751 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature