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Molecular Time Capsules Enable Transcriptomic Recording in Living Cells.

ABSTRACT: Live-cell transcriptomic recording can help reveal hidden cellular states that precede phenotypic transformation. Here we demonstrate the use of protein-based encapsulation for preserving samples of cytoplasmic RNAs inside living cells. These molecular time capsules (MTCs) can be induced to create time-stamped transcriptome snapshots, preserve RNAs after cellular transitions, and enable retrospective investigations of gene expression programs that drive distinct developmental trajectories. MTCs also open the possibility to uncover transcriptomes in difficult-to-reach conditions.

SUBMITTER: Parker M

PROVIDER: S-EPMC10614764 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Molecular Time Capsules Enable Transcriptomic Recording in Living Cells.

Parker Mirae M Rubien Jack J McCormick Dylan D Li Gene-Wei GW

bioRxiv : the preprint server for biology 20231016

Live-cell transcriptomic recording can help reveal hidden cellular states that precede phenotypic transformation. Here we demonstrate the use of protein-based encapsulation for preserving samples of cytoplasmic RNAs inside living cells. These molecular time capsules (MTCs) can be induced to create time-stamped transcriptome snapshots, preserve RNAs after cellular transitions, and enable retrospective investigations of gene expression programs that drive distinct developmental trajectories. MTCs ...[more]

PMID: 37905077

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