The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca².

ABSTRACT: Rotaviruses infect cells by delivering into the cytosol a transcriptionally active inner capsid particle (a "double-layer particle": DLP). Delivery is the function of a third, outer layer, which drives uptake from the cell surface into small vesicles from which the DLPs escape. In published work, we followed stages of rhesus rotavirus (RRV) entry by live-cell imaging and correlated them with structures from cryogenic electron microscopy and tomography (cryo-EM and cryo-ET). The virus appears to wrap itself in membrane, leading to complete engulfment and loss of Ca²⁺ from the vesicle produced by the wrapping. One of the outer-layer proteins, VP7, is a Ca²⁺-stabilized trimer; loss of Ca²⁺ releases both outer-layer proteins from the particle. The other outer-l

SUBMITTER: De Sautu M 

PROVIDER: S-EPMC10614792 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature