Blue light stimulated-autofluorescence green flash of lysosome-related organelles in the intestinal cells of nematodes.

ABSTRACT: The lysosome-related organelles ("gut granules") in the intestinal cells of many nematodes, including Caenorhabditis elegans, play an important role in metabolic and signaling processes, but they have not been fully characterized. We report here a previously undescribed phenomenon in which the autofluorescence of these granules displays a "flash" phenomenon in which fluorescence decreases are preceded by sharp increases in fluorescence intensity that expand into the surrounding area when the granules are stimulated with blue light. Autofluorescent granules are present in the intestinal cells of all six nematode species examined, with differences in morphology and distribution pattern. Five species exhibit the flash phenomenon: Panagrellus redivivus (Clade IV), Steinernema

SUBMITTER: Tan CH 

PROVIDER: S-EPMC10614822 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature