Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling.

ABSTRACT: Integrin-dependent adhesion to the extracellular matrix (ECM) mediates mechanosensing and signaling in response to altered microenvironmental conditions. In order to provide tissue- and organ-specific cues, the ECM is composed of many different proteins that temper the mechanical properties and provide the necessary structural diversity. Despite most human tissues being soft, the prevailing view from predominantly in vitro studies is that increased stiffness triggers effective cell spreading and activation of mechanosensitive signaling pathways. To address the functional coupling of ECM composition and matrix rigidity on compliant substrates, we developed a matrix spot array system to screen cell phenotypes against different ECM mixtures on defined substrate stiffnesses at high resolution.

SUBMITTER: Conway JRW 

PROVIDER: S-EPMC10614832 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature