Electrophysiological characterisation of iPSC-derived human β-like cells and an SLC30A8 disease model.

ABSTRACT: iPSC-derived human β-like cells (BLC) hold promise for both therapy and disease modelling, but their generation remains challenging and their functional analyses beyond transcriptomic and morphological assessments remain limited. Here, we validate an approach using multicellular and single cell electrophysiological tools to evaluate BLCs functions. The Multi-Electrode Arrays (MEAs) measuring the extracellular electrical activity revealed that BLCs are electrically coupled, produce slow potential (SP) signals like primary β-cells that are closely linked to insulin secretion. We also used high-resolution single-cell patch-clamp measurements to capture the exocytotic properties, and characterize voltage-gated sodium and calcium currents. These were comparable to those in primary β and EndoC-β

SUBMITTER: Jaffredo M 

PROVIDER: S-EPMC10614917 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature