The widely used <i>Ucp1-Cre</i>^<i>Evdr</i> transgene elicits complex developmental and metabolic phenotypes.

ABSTRACT: Bacterial artificial chromosome transgenic models, including most Cre-recombinases, enable potent interrogation of gene function in vivo but require rigorous validation as limitations emerge. Due to its high relevance to metabolic studies, we performed comprehensive analysis of the Ucp1-Cre^Evdr line which is widely used for brown fat research. Hemizygotes exhibited major brown and white fat transcriptomic dysregulation, indicating potential altered tissue function. Ucp1-Cre^Evdr homozygotes also show high mortality, growth defects, and craniofacial abnormalities. Mapping the transgene insertion site revealed insertion in chromosome 1 accompanied by large genomic alterations disrupting several genes expressed in a range of tissu

SUBMITTER: Halurkar MS 

PROVIDER: S-EPMC10614962 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature