Gene Expression Within a Human Choroidal Neovascular Membrane Using Spatial Transcriptomics.

ABSTRACT:

Purpose

Macular neovascularization is a relatively common and potentially visually devastating complication of age-related macular degeneration. In macular neovascularization, pathologic angiogenesis can originate from either the choroid or the retina, but we have limited understanding of how different cell types become dysregulated in this dynamic process.

Methods

To study how gene expression is altered in focal areas of pathology, we performed spatial RNA sequencing on a human donor eye with macular neovascularization as well as a healthy control donor. We performed differential expression to identify genes enriched within the area of macular neovascularization and used deconvolution algorithms to predict the originating cell type of these dysregulated genes.

Results

SUBMITTER: Voigt AP 

PROVIDER: S-EPMC10615143 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature