Cyasterone has a protective effect on steroid-induced Osteonecrosis of the femoral head.

ABSTRACT:

Context

Cyasterone alleviated the apoptosis of BMSCs induced by Dexamethasone via the PI3K/AKT signaling pathway. In addition, Cyasterone had a protective effect on SIONFH model rats by reducing the percentage of empty bone lacunae.

Objective

To study the effect of Cyasterone on apoptosis of rat BMSCs and its function on the SIONFH rat model.

Methods

Rat BMSCs were cultured and divided into Control, DXM and Cyasterone (DXM+Cyasterone) groups. The apoptosis of each group was detected by flow cytometry, the expressions of Caspase-3 and Caspase-9 were detected by immunofluorescence staining, and the mRNA and protein expressions of AKT, BAX, P53, P85, Bcl-2 and Cytochrome C were detected by qPCR and WB. In animal experiments, the femoral head of rats were subjected to HE

SUBMITTER: Sun Y 

PROVIDER: S-EPMC10615314 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature