Analysis of human immunodeficiency virus cytopathicity by using a new method for quantitating viral dynamics in cell culture.

ABSTRACT: Human immunodeficiency virus (HIV) causes complex metabolic changes in infected CD4(+) T cells that lead to cell cycle arrest and cell death by necrosis. To study the viral functions responsible for deleterious effects on the host cell, we quantitated the course of HIV type 1 infection in tissue cultures by using flow cytometry for a virally encoded marker protein, heat-stable antigen (HSA). We found that HSA appeared on the surface of the target cells in two phases: passive acquisition due to association and fusion of virions with target cells, followed by active protein expression from transcription of the integrated provirus. The latter event was necessary for decreased target cell viability. We developed a general mathematical model of viral dynamics in vitro in terms of three effectiv

SUBMITTER: Speirs C 

PROVIDER: S-EPMC1061548 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature