E-cadherin adhesion dynamics as revealed by an accelerated force ramp are dependent upon the presence of α-catenin.

ABSTRACT: Tissue remodeling and shape changes often rely on force-induced cell rearrangements occurring via cell-cell contact dynamics. Epithelial cell-cell contact shape changes are particularly dependent upon E-cadherin adhesion dynamics which are directly influenced by cell-generated and external forces. While both the mobility of E-cadherin adhesions and their adhesion strength have been reported before, it is not clear how these two aspects of E-cadherin adhesion dynamics are related. Here, using magnetic pulling cytometry, we applied an accelerated force ramp on the E-cadherin adhesion between an E-cadherin-coated magnetic microbead and an epithelial cell to ascertain this relationship. Our approach enables the determination of the adhesion strength and force-dependent mobility of individual a

SUBMITTER: Bush J 

PROVIDER: S-EPMC10615569 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature