Alloantigen Infusion Activates the Transcriptome of Type 2 Conventional Dendritic Cells.

ABSTRACT: Recent studies have revealed novel molecular mechanisms by which innate monocytic cells acutely recognize and respond to alloantigen with significance to allograft rejection and tolerance. What remains unclear is the single-cell heterogeneity of the innate alloresponse, particularly the contribution of dendritic cell (DC) subsets. To investigate the response of these cells to exposure of alloantigen, C57BL/6J mice were administered live allogenic BALB/cJ splenic murine cells versus isogenic cells. In parallel, we infused apoptotic allogenic and isogenic cells, which have been reported to modulate immunity. Forty-eight hours after injection, recipient spleens were harvested, enriched for DCs, and subjected to single-cell mRNA sequencing. Injection of live cells induced a greater transcripti

SUBMITTER: Schroth SL 

PROVIDER: S-EPMC10615655 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature