Microarray analysis of hub genes, non-coding RNAs and pathways in lung after whole body irradiation in a mouse model.

ABSTRACT:

Purpose

Previous research has highlighted the impact of radiation damage, with cancer patients developing acute disorders including radiation induced pneumonitis or chronic disorders including pulmonary fibrosis months after radiation therapy ends. We sought to discover biomarkers that predict these injuries and develop treatments that mitigate this damage and improve quality of life.

Materials and methods

Six- to eight-week-old female C57BL/6 mice received 1, 2, 4, 8, 12 Gy or sham whole body irradiation. Animals were euthanized 48 h post exposure and lungs removed, snap frozen and underwent RNA isolation. Microarray analysis was performed to determine dysregulation of messenger RNA (mRNA), microRNA (miRNA), and long non-coding RNA (lncRNA) after radiation injury.

Result

SUBMITTER: Aryankalayil MJ 

PROVIDER: S-EPMC10615684 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature