Decellularized human pancreatic extracellular matrix-based physiomimetic microenvironment for human islet culture.

ABSTRACT: A strategy that seeks to combine the biophysical properties of inert encapsulation materials like alginate with the biochemical niche provided by pancreatic extracellular matrix (ECM)-derived biomaterials, could provide a physiomimetic pancreatic microenvironment for maintaining long-term islet viability and function in culture. Herein, we have demonstrated that incorporating human pancreatic decellularized ECM within alginate microcapsules results in a significant increase in Glucose Stimulation Index (GSI) and total insulin secreted by encapsulated human islets, compared to free islets and islets encapsulated in only alginate. ECM supplementation also resulted in long-term (58 days) maintenance of GSI levels, similar to that observed in free islets at the first time point (day 5). At ear

SUBMITTER: Asthana A 

PROVIDER: S-EPMC10615794 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature