Microfluidic single-cell migration chip reveals insights into the impact of extracellular matrices on cell movement.

ABSTRACT: Cell migration is a complex process that plays a crucial role in normal physiology and pathologies such as cancer, autoimmune diseases, and mental disorders. Conventional cell migration assays face limitations in tracking a large number of individual migrating cells. To address this challenge, we have developed a high-throughput microfluidic cell migration chip, which seamlessly integrates robotic liquid handling and computer vision to swiftly monitor the movement of 3200 individual cells, providing unparalleled single-cell resolution for discerning distinct behaviors of the fast-moving cell population. This study focuses on the ECM's role in regulating cellular migration, utilizing this cutting-edge microfluidic technology to investigate the impact of ten different ECMs on triple-negative

SUBMITTER: Zhou M 

PROVIDER: S-EPMC10615797 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature